Professor and Department Chair
Division of Periodontology
Department of Developmental and Surgical Sciences
BA, Minnesota State University, 1970
Thesis: The radiation resistance of Micrococcus radiodurans, MS, Minnesota State University, 1971
Thesis: Studies on a bactericidal substance produced by Group A Streptococci, Ph.D., Northwestern University Medical and Dental School, 1974
Dentistry, Doctor of Dental Surgery (DDS), University of Minnesota School of Dentistry, 1978 - Periodontics, Certificate, New York University Dental Center, 1980
17-164 Moos Tower
515 Delaware St. SE
Minneapolis, MN 55455
Dr. Larry Wolff is a graduate of the University of Minnesota School of Dentistry. He received his PhD in Microbiology at Northwestern University and his certificate in Periodontics from New York University Dental Center. Dr. Wolff has been a full-time faculty member at the University of Minnesota School of Dentistry since 1980 and currently is Professor and Chair in the Department of Developmental and Surgical Sciences. He has lectured extensively at the National and International levels in areas related to the use of antimicrobial agents as adjuncts to periodontal therapy and systemic conditions as related to periodontal disease. Dr. Wolff has numerous publications on topics related to the clinical use of antimicrobials as adjuncts to periodontal treatment in dentistry and periodontics and in addition, has published extensively in the area of periodontal microbiology.
Dr. Wolff teaches and pursues research interests in the field of periodontology. His basic research interest focuses on the bacterial etiology of periodontal diseases, new diagnostic techniques to identify bacteria in plaque, the role of cytokines on the periodontal disease process, and the expression of virulence factors by periodontopathic bacteria and the host response to those virulence factors. On the clinical side, he's currently pursuing studies of antimicrobial therapy for the treatment of periodontal disease and is also exploring the relationship between periodontal disease and a variety of systemic conditions, including cardiovascular disease and diabetes. In the area of antimicrobial therapy, Dr. Wolff studies the role of local delivery of antimicrobial therapy and systemic antibiotics as adjuncts in treating periodontal disease. Other areas of current research interest include the effect of smoking on periodontal disease and as a risk factor for progressive periodontitis, subgingival temperature as a diagnostic aid, and the effect of sonic toothbrushes in reducing plaque and gingival inflammation around implants.
Professor of the Year Award (In recognition of outstanding teaching, service and support to the School of Dentistry/University of Minnesota from the Class of 2013) 2012
Clinical Impact Award (Recognition by the American Academy of Periodontology of Research having the greatest potential for clinical applicability, Tissue Engineered Cultured Periosteal Sheet Application to Periodontal Regeneration: Three Year Results) 2011
Professor of the Year Award (In recognition of outstanding teaching, service and support to the School of Dentistry/University of Minnesota from the Class of 2012.) 2011
Lecturer of the Year Award (In recognition of outstanding teaching, service and support to the School of Dentistry/University of Minnesota from the Class of 2011) 2010
Induction into the American College of Dentists 2006
Outstanding Lecturer of the Year 2004
Century Club Professor of the Year 2003
Inducted into Pierre Fauchard Academy 2003
Inducted into the International Association of Dentists 2002
Dental Instructor of the Year, Presented by Second Year Class, University of Minnesota School of Dentistry 1990
Clinical Research Award 1988
Outstanding Lecturer of the Year, Presented by the third year class, University of Minnesota School of Dentistry 2004
Omicron Kappa Upsilon - Honorary Dental Society 1984
Instructor of the Year, Presented by the Second Year Class, University of Minnesota School of Dentistry 1982
Outstanding Lecturer of the Year, Presented by the Second Year Class, School of Dentistry, University of Minnesota 1981
Okuda, K., Kawase, T., Nagata, M., Yamamita, K., Nakata, K., Wolff, L., Yoshie, H. Five Year Results of Tissue Engineered Cultured Periosteal Sheet Application to Periodontal Regeneration: Case Series. The International Journal of Periodontics and Restoratie Dentistry. In press. 2012.
Kamiya, M., Kawase, T., Kobayashi, M., Sekine, Y., Okuda, K., Nagata, M., Fuse, I., Nakata, K., Wolff, L., and Yoshie, H. A short-term preservation of human cultured periosteal sheets, osteogenic grafting materials, using a commercial preservation solution containing epigallocatechin-3-gallate (Theliokeep®) under chilled conditions. Journal of Biopreservation and Biobanking, 2012; 10(3):245-252.
Nakayama, H., Kawase, T., Okuda, K., Wolff, L., and Yoshie, H. In-vivo near-infrared optical imaging of growing osteosarcoma cell lesions xenographed in mice: dual channel qualitative evaluation of volume and mineralization. Acta Radiogica, 2011; 52:978-988.
Nakajima, Y., Kawase, T., Kabayashi, M., Okuda, K., Wolff, L., and Yoshie, H. Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material. Platelets, in press, 2012.
Shirai, S., Okuda, K., Kubota, T., Wolff, L., and Yoshie, H. The comparative effectiveness of granules or blocks of superpourous hydroxyapatite for the treatment of intrabony periodontal defects. Open Journal of Stamatology, 2012; 02:81-87.
Kawase, T., Okuda, K., Wolff, L., Yoshie, H. Collagen Coated Film: A Promising Scaffold for Cultured Periosteal Sheets. Journal of Periodontology, 81:1653-1662, 2010.
Nakayama, H., Kawase, T., Kogami, H., Okuda, K., Inoue, H., Oda, T, Hayama, K., Tsuchimochi, M., and Wolff, L. Evaluation by Bone Scintigraphy of Osteogenic Activity of Commercial Bioceramics (Porous -TCP and HAp Particles) Subcutaneously Implanted in Rats. J. Biomaterials Applications. 24:751-68, 2010.
Wolff, R., Wolff, L., Michalowicz, B. A Pilot Study of the Relationship Between Periodontitis and Glycated Hemoglobin Levels in Non-Diabetics. J. Periodontol 80:1057-1061, 2009.
Yamamiya, K., Okuda, K., Kawase, T., Wolff, L. and Yoshie, H. Tissue engineered cultured periosteum used with platelet-rich plasma and hydroxyapatite in treating human osseous defects. J. Periodontol. 74:811-818, 2008.
Murata, M., Okuda, K., Momose, M., Wolff, L. and Yoshie, H. Root coverage with cultured grafted dermal substitute composed of gingival fibroblast and matrix. Int J Periodontol and Restor Dent, 28:461-467, 2008.
Okuda, T., Tai, H., Tanabe, K., Suzuki, H, Sato, T., Kawase, T., Saito, Y., Wolff, L., Yoshie, H. Platelet-rich plasma combined with a porous hydroxyapatite graft for the treatment of intrabony periodontal defects in humans: A comparative controlled clinical trial. J. Periodontol. 76:890-898, 2005.
Knorr, S., Combe, E., Wolff, L., Hodges, J. The surface free energy of gold alloy systems. Dental Materials. 21: 272-277, 2005.
Alpagot, T., Wolff, L., et al. Risk factors for periodontitis in HIV positive patients. J Periodont Res. 39:149-157, 2004.
Martorelli de Lima A.F., Curl C.C., Palioto D.B., Guro A.M., Silva R.C., and Wolff, L.F. Therapy with adjunctive doxycycline local delivery in patients with type I diabetes mellitus and periodontitis. J. Clin Periodontol. 31:648-653, 2004.
Molloy, J., Wolff, L., Guzman, A.L., Hodges, J. The relationship of periodontal disease parameters with systemic medical conditions and tobacco use. J. Clin Periodontol. Accepted for publication, 2004.
Okuda, K., Momose, M., Wolff, L. et al. Treatment of chronic desquamative gingivitis using tissue engineered human cultured gingival epithelial sheets. Int'l. J. of Periodon. & Restorative Dentistry. 24: 119-125, 2004.
Okuda, K., Kawase, T., Wolff, L. et al. Platelet rich plasma contains higher levels of PDGF and TGF-b and modulates the proliferation of periodontal related cells in vitro. J. Periodontol. 74:849-857, 2003.
Kawase, T., Okuda, K., Wolff, L. et al. Platelet rich plasma derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoplastic cells in vitro. J. Periodontol. 74:858-864, 2003.
Shelburne, C.F., Gleason, R., Germaine, G., Wolff, L., Mullally, B., Coulter, W. and Lopatin, D. Quantitative Reverse Transcription Polymerase Chain Reaction (QRT-PCR) analysis of Porphyromonas Gingivalis Gene Expression in vivo. J. Microbiological Methods. 49:147-156, 2002.
Tsai, Ching Yin, Wolff, L., Germaine, G., and Hodges, J. A Rapid DNA Probe Test Compared to Culture Methods for Identification of Subgingival Plaque Bacteria. J. Clin. Periodontol. 30(1):57-62, 2003.
Williams, R., Wolff, L. et al. Treatment of Periodontitis by Local Administration of Minocycline Microspheres: A controlled trial. J. Periodontology, 72:1535 -1544, 2001.
Okuda, K., Wolff, L. et al. Levels of tissue Inhibitor of Metalloproteinaises-1, matrix metalloproteinalises-1 and -8 levels in gingival Crevicular Fluid Following Treatment with Enamel Matrix Derivative (Emdogain). J. Periodont. Res. 36: 309-316, 2001.
Chen, X., Wolff, L. and Aeppli, D. Cigarette smoking, salivary/gingival crevicular fluid cotinine and periodontal status. A ten-year longitudinal study. J. Clin. Periodontol. 28: 331-339, 2001.
Henke, C., Wolff, L. et al. Economic evaluation of a chlorhexidine chip for treating adult periodontitis. JADA. 132: 1557-1569, 2001.
Mullally, B, Wolff, L. et al. Prevalence of Periodontal Pathogens in Early Onset Periodontitis. J. Periodont. Res. 35: 232-241, 2000.
Okuda, K., Wolff, L. et al. Enamel matrix derivatives (Emdogain) in the treatment of human infrabony periodontal osseous defects. J. Periodont. 71:1821-1828, 2000.
Wolff, L. and Mullally, B. New clinical materials and techniques in guided tissue regeneration. International Dental Journal. 50: 235-244, 2000.
Michalowicz, B., Wolff, L., Hinrichs, J., Aeppli, D., Pihlstrom, B. Periodontal bacterial pathogens in adult twins. Periodontol. 70:263-273, 1999.
Wolff, L., Kim, A., Bakdash, B., and Nunn, M. Efficacy of a sonic toothbrush in maintenance of dental implants: A prospective study. J. Clin. Periodontol. 25:821-828, 1998.
Wolff, L., Koller, N., Smith, Q., Mathur, A. and Aeppli, D. Subgingival Temperature: Relation to gingival crevicular fluid enzymes, cytokines and subgingival plaque microorganisms. J. Clin. Periodontol. 24:900-906, 1997.
Wolff, L. F. Risk factors for progressive periodontitis and treatment of periodontitis through local drug delivery. Niigata Dent. J. 27 (1):49-55, 1997.
Wolff, L.; Kohler, N; Smith, Q and Aeppli, D. Subgingival temperature: Relationship to crevicular enzymes, cytokines and plaque microorganisms. J. Clin. Periodontol. 24: 900-906, 1997.
Tseng, CC and Wolff, LF. Simultaneous team approach of a gingival-lengthening procedure and an operative restoration. Quintessence International, 28:249-254, 1997.
Alpagot, T.; Wolff, L.; Smith, Q., Tran, S., Aeppli, D. and Hardie, N. Risk indicators for periodontal disease in a racially diverse urban population. J. Clin. Periodontology. 23:982-988, 1996
O'Brien, T., Roszkowski, M., Wolff, L. and Hargreaves, K. The effect of Ibuprofen on tissue levels of iPGE2, iLTB4 and pain after periodontal surgery. J. Periodontology. 67:1307-1316, 1996.
Wolff, L.F., Dahlen, G. and Aeppli, D. Bacteria as risk markers for periodontitis. J Periodontology 64:498-510, 1994.
Mullally, B., Wolff, L., Hardie, N., Aeppli, D., Pihlstrom, B. The effect of gingival fluid collection on subgingival plaque sampling. J of Dentistry, 22:223-228, 1994.
Stoltenberg, J.L., Osborn, J.B., Pihlstrom, B.L., Herzberg, M.C., Aeppli, D.M., Wolff, L.F. and Fischer, G.E. Association between cigarette smoking, bacterial pathogens and periodontal status. J. Periodontol. 64:1225-1231, 1994.
Tervonen, T., Oliver, R. C., Wolff, L.F., Bereuter, J., Anderson, L., Aeppli, D.M. Prevalence of periodontal pathogens in diabetic patients with special reference to control of diabetes mellitus. J of Clinical Periodontology 21:375-379, 1994.
Wolff, L.F., Aeppli, D.M., Pihlstrom, B.L., Anderson, L., Stoltenberg, J.L., Osborn, J.B., Hardie, N.A., Shelburne, C.E. and Fischer, G.E. Natural distribution of five bacteria associated with periodontal disease. J Clinical Periodontology, 20:699-706, 1993.
Shelburne, C.E., Sandberg, G.P., Binsfeld, C.A., Wolff, L.F. and Curry, R.A. Monoclonal antibodies to lipopolysaccharide of four oral bacteria associated with periodontal disease. J. Periodontal Research, 28:1-9, 1993.
Liljemark, W.F., Bloomquist, C. G., Bandt, C.L., Pihlstrom, B.L., Hinrichs, J.E. and Wolff, L.F. Comparison of the distribution of Actinomyces in denta plaque on inserted enamel and natural tooth surfaces in periodontal health and disease. Oral Biology and Immunology 8(1):5-15, 1993.
Okuda, K., Wolff, L.F., Oliver, D.O., Bereuter, J., Aeppli, D.A. and Hara, K. Effect of a monocycline slow-release formulation on subgingival bacteria. J. Periodontology, 63:73-79, 1992
MacFarlane, G.D., Herzberg, M.C., Wolff, L.F. and Hardie, N.A. Refractory periodontitis associated with abnormal polymorphonuclear leukocyte phagocytosis and cigarette smoking. J. Periodontol. 64:908-913, 1992
Abramson, M., Wolff, L.F. and Offenbacher, S.O. Flurbiprofen effect on prostaglandin/thromboxane levels in human crevicular fluid. J Periodontal Research, 27:539-543, 1992.
Wolff, L.F., Anderson, L., Sandberg, G., Aeppli, D. and Shelburne, C. Bacterial Concentration Fluorescence Immunoassay (BCFIA) for detecting periodontopathogens in plaque. J.Periodontology, 63:1093-1101, 1992.
Tseng, C.C., Wolff, L.F. and Aeppli, D.M. Effect of gels containing stannous fluoride on oral bacteria. An in vitro study. Australian Dental Journal, 37(5):368-73, 1992.
Skaar, D.D., Wolff, L.F., Aeppli, D.M., Bloomquist, C.G. and Liljemark, W.F. A follow-up case report of Actinobacillus actinomycetemcomitans in human periodontal disease. J. Clinical Periodontology, 19:288-2291, 1992.
Wolff, L.F., Anderson, L., Sandberg, G.P., Aeppli, D.M. and Shelburne, C.E. A Fluorescence Immunoassay for Detecting Periodontal Bacterial Pathogens in Plaque. J.Clinical Microbiology, 29:1645-1651, 1991.
Tseng, C.C. and Wolff, L.F. Inhibitory effect of stannous fluoride and other commonly used antimicrobial agents on oral bacterial. J.Formosan Medical Association, 90:565-571, 1991.
Tseng, C.C. and Wolff, L.F., The periodontal status of patients with Sjogren's Syndrome. J Clinical Periodontology, 17:329-330, 1990.
Wolff, L.F., Anderson, L., Sandberg, G., Shelburne, C. Particle concentration immunofluorescent assay for bacterial detection in periodontal disease. In Proceedings of the 1990 Pandex Users Symposium on Particle Concentration Fluorescence Immunoassay, TPBT Inc. pp 12-15, 1990.
Cao, C.F., Aeppli, D.M., Liljemark, W.F., Bloomquist, C.G., Bandt, C.L. and Wolff, L.F. Comparisons of plaque microflora between Chinese and Caucasian population groups. J. Clinical Periodontology, 17:115-118, 1990.
Tseng, C.C. and Wolff L.F. Current status of stannous fluoride use in periodontics. J. Dental Science. 9:141-146, 1989.
Wolff, L.F., Pihlstrom, BL, Bakdash, MB, Schaffer, EM, Aeppli, DM, and Bandt, CL. Four-year investigation of salt and peroxide regimen compared with conventional oral hygiene. J. American Dental Association. 118:67-72, 1989.
Wolff, L.F., Pihlstrom, BL, Bakdash, MB, Aeppli, DM, and Bandt, CL. Effect of toothbrushing with 0.4% stannous fluoride and 0.22% sodium fluoride gel on gingivitis over eighteen months. J. American Dental Association, 119:283-289, 1989.
Wolff, L.F., Bakdash, M.B., Pihlstrom, B.L., Bandt, C.L. The effect of professional and home subgingival irrigation with antimicrobial agents on inflammatory periodontal disease. J. Dental Hygiene, 63:222-227, 1989.
Wolff, L.F., Liljemark, W.F., Pihlstrom, B.L., Schaffer, E.M., Aeppli, D.M., Bandt, C.L. Dark-pigmented Bacteroides species in subgingival plaque of adult patients on a rigorous recall program. J. Periodontal Research 23:170-174, 1988.
Wolff, L.F., Smith, Q.T., Snyder, W.S., Bedrick, J.A., Liljemark , W.F., Bandt, C.L. Relationship between lactate dehydrogenase levels in human gingival fluid with clinical and microbial measurements. J. Clinical Periodontology 15:110-115, 1988.
Pihlstrom, B.L., Wolff, L F, Bakdash, M.B., Schaffer, E.M., Jensen, J.R., Jr., Aeppli, D.M., Bandt, C.L. Salt and peroxide compared to conventional oral hygiene. I. Clinical results. J. Periodontology 58:291-300, 1987.
Wolff, L.F., Pihlstrom, B.L., Bakdash, M.B., Schaffer, E.M., Jensen, J.R. Jr., Aeppli, D.M., Bandt, C.L. Salt and peroxide compared to conventional oral hygiene. II. Microbial results. J. Periodontology 58:301-307, 1987.
Bakdash, M.B., Wolff, L.F., Pihlstrom, B.L., Aeppli, D.M., Bandt, C.L. Salt and peroxide compared to conventional oral hygiene. III. Patient compliance and acceptance. J.Periodontology 58:308-313, 1987.
Wolff, L.F., Bakdash, M. B., Bandt, C.L. Microbial interpretation of plaque relative to the diagnosis and treatment of periodontal disease. J. Periodontology 56:281-284, 1985.
Pihlstrom, B.L., Liljemark, W.F., Schaffer, E.M., Wolff, L.F., Smith, L.A., Bandt, C.L. The relationships of probing depth and total microscopic counts to differential subgingival plaque morphology. J. Periodontal Research 20:106-112, 1985.
Hinrichs, J.E., Wolff, L.F., Pihlstrom, B.L., Schaffer, E.M., Liljemark, W.F., Bandt, C.L. Effects of scaling and root planing on subgingival microbial proportions standardized in terms of their naturally occurring distribution. J. Periodontology 56:187-194, 1985.
Wolff, L.F., Liljemark, W.F., Bloomquist, C.G., Pihlstrom, B.L., Schaffer, E.M., Bandt, C.L. The distribution of Actinobacillus actinomycetemcomitans in human plaque. J. Periodontal Research 20:237-250, 1985.
Wolff, L.F., Krupp, M.J., Liljemark, W.F. Microbial changes associated with advancing periodontitis in STR/N mice. J. Periodontal Research 20:378-385, 1985.
Wolff, L.F., Pihlstrom, B.L., Liljemark, W.F., Schaffer, E.M., Bandt, C.L. Distinct categories of microbial forms associated with periodontal disease. J Periodontal Research 20:497-502, 1985.
Liljemark, W.F., Bloomquist, C.G., Uhl, L.A., Schaffer, E.M., Wolff, L.F., Pihlstrom, B.L., Bandt, C.L. The distribution of oral Haemophilus species in dental plaque from a large adult population. Infection and Immunity 46:778-786, 1984.
Wolff, L.F., Bandt, C.L., Pihlstrom, B.L., Brayer, L. Phase contrast microscopic evaluation of subgingival plaque in combination with either conventional or antimicrobial home treatment of patients with periodontal inflammation. J. Periodontal Research 17:537-540, 1982.
Bandt, C.L., Liljemark, W.F., Pihlstrom, B.L., Hinrichs, J.E., Schaffer, E.M., Wolff, L.F. The use of selective media for examining relationships between dental plaque microorganisms and periodontal disease in a large sample clinical investigation. J. Periodontal Research 17:518-520, 1982.
Everhart, D.L., Dahab, O., Wolff, L.F., Stahl, S.S. The further localization of antibody on cemental tissue. J. Periodontology 53:168-171, 1982.
Wolff, L.F., Liljemark, W.F. Observations of beta-hemolysis among three strains of Streptococcus mutans. Infection and Immunity 19:745-748, 1978.
Wolff, L.F., Duncan, J.L. Studies on a bactericidal substance produced by Group A Streptococci. J. Microbiology 81:413-424, 1974.