Paul J. Jardine, PhD

Associate Professor, Department of Diagnostic and Biological Sciences

Paul J. Jardine

Contact Info

jardine@umn.edu

Office Phone 612-624-0667

Fax 612-625-1108

Office Address:
Diag/Biolo Sci - Molecular Virology
18-2462 Moos Tower
515 Delaware St SE
Minneapolis, MN 55455

Mailing Address:
School of Dentistry
Room 15-229 Moos Tower
1291A
515 Delaware St SE
Minneapolis, MN 55455

Administrative Assistant Name
Sue Kostka

Administrative Email
kostk008@umn.edu

PhD, University of New Brunswick (Virology)

BS, University of New Brunswick, (Biology) 1990

Diploma in University Teaching, University of New Brunswick Teaching Centre, 1996

Summary

As the AHC Course Director for Foundations of Interprofessional Communication and Collaboration (FIPCC), Dr. Jardine is responsible for this introductory course in the AHC 1Health curriculum. As Director, his role is to consult with the schools and colleges of the AHC in the development and implementation of FIPCC, which serves as the Phase I introduction to interprofessional education and training.

Dr. Jardine’s primary appointment is as a Research Associate Professor in the School of Dentistry, where he studies virus structure and assembly and teaches microbiology. He has been involved in both interdisciplinary teaching and research throughout his career. He earned a Diploma in University Teaching from the University of New Brunswick, Canada, where he acted as a facilitator in graduate level interdisciplinary courses in science philosophy and research design. At the University of Minnesota School of Dentistry, he has been a facilitator in professional ethics courses, as well as the course pilots of FIPCC. As part of his research efforts studying biological molecular motors, he is part of a broad, interdisciplinary research team that brings together the fields of virology, biochemistry, genetics, and biophysics.

Awards & Recognition

  • National Science and Engineering Research Council (Canada) Postdoctoral Fellowship in Biophysics, 1999-2000

Research

Research Summary/Interests

Virus structure and assembly; Biochemistry, Biophysics and Structural Biology; Interprofessional Education My interests revolve around the investigation of viral assembly and the description of biological motors at the molecular level. Both historically and experimentally, bacteriophages provide a perfect system to address both of these issues. My current focus is on the study of bacteriophage phi29 DNA packaging. A molecular motor assembles transiently to the phi29 prohead capsid and packages DNA to near crystalline density, powered by ATP hydrolysis. To fully describe the mechanism of this motor, and gain understanding about molecular motors in general, I am part of a large interdisciplinary group comprised of specialists in structural biology, biophysics, molecular genetics and biochemistry. As a phage biochemist, my role is to translate the basic components of phi29 packaging from our robust in vitro packaging system into hands of specialist who can address the questions of interest our work has generated, as well as develop new approaches meant to interrogate the packaging mechanism.

Research Funding Grants

Research Grant, Academic Health Center Faculty Research Development Grant, (PI), Title - Structural Dynamics of a Motor Involved in Virus Assembly. University of Minnesota, 2003-2005.

Research Grant, NIH/NIDCR RO1, (co-PI), Title - Morphogenesis of Bacteriophage phi29. University of Minnesota, 2003-2008.

Research Grant, NIH/NIGMS RO1, (co-PI), Title - RNA in Viral DNA Packaging. University of Minnesota, 2004-2008.

Research Grant, NIH/NIGMS RO1, (co-PI), Title - Mechanisms of Viral DNA Packaging. UC Berkeley, UC San Diego and University of Minnesota, 2004-2008.

Post-doctoral Fellowship, University of Minnesota, 1997-98. 

NSERC Post-doctoral Fellowship (Biophysics), University of New Brunswick, 1999-2000.

Research Grant, Interdisciplinary Research and Postbaccalaureate Education Program, (PI), University of Minnesota, 1998.

Research Grant, Grant-In-Aid of Research, Artistry and Scholarship, (PI),
University of Minnesota, 2001-03.

Publications

  • Jardine, P. J. and D. Anderson. 2005. DNA packaging in double-stranded DNA bacteriophages. The Bacteriophages, R. Calendar, ed., Oxford Press, in press
  • Guasch, A., J. Pous, B. Ibarra, F. X. Gomis-R?th, J. M. Valpuesta, N. Sousa, J. L. Carrascosa, M. Coll, A. A. Simpson, Y. Tao, P. G. Leiman, M. O. Badasso, Y. He, P. J. Jardine, N. H. Olson, M. C. Morais, S. Grimes, D. L. Anderson, T. S. Baker, and M. G. Rossmann. 2002. Note to the Paper by Guasch et al. (2002) Detailed Architecture of a DNA Translocating Machine: The High-resolution Structure of the Bacteriophage Connector Particle. J. Mol. Biol. 321(2):379-80.
  • Grimes, S., P. J. Jardine, and D. Anderson. 2002. Bacteriophage DNA packaging. Adv. Virus Res. 58:255-294.
  • Morais, M. C., Y. Tao, N. H. Olson, S. Grimes, P. J. Jardine, D. L. Anderson, T. S. Baker, and M. G. Rossmann. 2001. Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage. J. Struct. Biol. 135(1):38-46.
  • Simpson, A. A., P. G. Leiman, Y. Tao, Y. He, M. O. Badasso, P. J. Jardine, D. L. Anderson, and M. G. Rossmann. 2001. Structure determination of the head-tail connector of bacteriophage. Acta Cryst. D57:1260-1269.
  • Simpson, A. A., Y. Tao, P. G. Leiman, M. O. Badasso, Y. He, P. J. Jardine, N. H. Olson, M. C. Morais, S. Grimes, D. L. Anderson, T. S. Baker, and M. G. Rossmann. 2000. Structure of the bacteriophage ?29 DNA packaging motor. Nature 408:745-750.
  • Jardine, P. J. and D. H. Coombs. 1998. Capsid expansion follows the initiation of DNA packaging in bacteriophage T4. J. Mol. Biol. 284(3):661-72.
  • Jardine, P. J., M. C. McCormick, C. Lutze-Wallace, D. H. Coombs. 1998. The bacteriophage T4 DNA packaging apparatus targets the unexpanded prohead. J. Mol. Biol. 284(3):647-59.

Teaching

Academic Interests and Focus

Virology; Biochemistry

Courses

Dent5303, Microbiology for Dental Students, 2001-present - core course in microbiology for DDS students Dent5301, Introduction to Oral Biology, 2003, 2005 - seminar course in scientific literature review for DDS t5412, Professional Problem Solving, 2005 - seminar course in professional ethics for DDS students