Kim Mansky, PhD

Professor, Division of Orthodontics

Kim Mansky

Contact Info

kmansky@umn.edu

Office Phone 612-626-5582

Fax 612-626-2571

Lab Phone 612-624-0986

Office Address:
Develomental/Surgical Sci-Orthodont
Room 16-146 MoosT
515 Delaware St SE
Minneapolis, MN 55455

Mailing Address:
School of Dentistry
Room 15-229 MoosT
1291A
515 Delaware St SE
Minneapolis, MN 55455

Postdoctoral fellowship, Ohio State University (Bone biology)

PhD, University of Wisconsin, Madison (Oncology) 1997

BS, University of North Carolina (Biology) 1991

Summary

Dr. Mansky earned her Bachelor's degree in biology from the University of North Carolina in Chapel Hill in 1991. She worked as a technician at the University of North Carolina from 1991-1992. She completed her Ph.D. degree at University of Wisconsin-Madison in 1997 with a focus on oncology with the study of papillomaviruses. She then moved to Ohio State University in Columbus, Ohio to pursue a postdoctoral fellowship in bone biology with an emphasis on osteoclast differentiation. Dr. Mansky accepted a faculty position at the University of Minnesota in 2003, and she continues to further her efforts in trying to understand osteoclast differentiation.

Research

Research Summary/Interests

Osteoclast differentiation, epigenetic regulators, estrogen signaling, mandible derived osteoclasts, and myokines

Bone is often thought to be a tissue that does not change once it is formed; however, bone is very dynamic and is constantly being remodeled. In healthy individuals, bone formation and resorption are in balance. Osteoporosis, which affects ten million people in the United States and puts at risk another thirty-four million Americans, is a result of the uncoupling of bone formation and resorption. Osteoclasts are the bone cells responsible for resorption. Characterizing molecular mechanisms that regulate osteoclast differentiation have become crucial because of the substantial morbidities and associated public health costs of uncontrolled bone destruction. Combinations of epigenetic modifications are proposed to coordinate gene expression during cellular differentiation. In cells other than osteoclasts, proteins that regulate methylation and acetylation, two common epigenetic modifications, have been shown to regulate multiple cellular functions. The Mansky lab is currently investigating the role of various epigenetic proteins and their role in regulating osteoclast differentiation. Epigenetic inhibitors are being tested separately and in combination clinically to treat various diseases; therefore, it is critical to characterize their role in osteoclast differentiation to determine the effect on skeletal health.

The myocyte enhancer protein 2 (MEF2) family of transcription factors are a common target of histone deacetylases (HDACs). HDACs are a group of epigenetic regulators that deacetylate chromatin to inhibit gene expression. Female mice null for MEF2A in osteoclasts had increased bone volume/total volume; however, male mice null for MEF2A in osteoclasts had no significant skeletal phenotype. In culture, osteoclasts null for MEF2A are smaller and have less activity compared to osteoclasts isolated from wild type mice. In cardiac cells MEF2A and HDACs have been shown to regulate estrogen signaling. Estrogen signaling is a negative regulator of osteoclast differentiation and activity. Currently the Mansky lab is investigating the role of MEF2A and HDACs in regulating estrogen signaling during osteoclast differentiation.

Bones in the mandible and the maxilla are developmentally and functionally distinct from the bones in the appendicular skeleton. Studies from transgenic mouse models demonstrate bone site specific osteopetrosis. In these mouse models, the osteopetrotic skeleton does not always correlate with a loss of tooth eruption suggesting that regulation of osteoclast activities in the jaw and the rest of the skeleton differ. Preliminary studies from the Mansky lab in collaboration with Dr. Amy Tasca have confirmed osteoclasts from the marrow of the mandible are larger in size compared to osteoclasts derived from the marrow of the femur. We are currently conducting studies to characterize the properties of jaw osteoclasts compared to femur osteoclasts.

Publications

  • Blixt, N. Norton, A., Zhang, Anqi, Aparicio, Conrado, Prasad, Hari, Gopalakrishan, Rajaram, Jensen, Eric and
  • Mansky, Kim. 2020 Loss of MEF2 expression in osteoclasts leads to sex-specific and osteopenic phenotypes. Bone accepted 6/1/20.
  • Blixt, N. Faulkner, B.K, Astleford, K., Lelich, R., Schering, J., Spencer, E., Gopalakrishnan, R., Jensen, E.D., and Mansky, K.C. 2017 Class II and IV HDACs Function as Inhibitors of Osteoclast Differentiation PLOS ONE, Sept 27; 12(9): e0185441 PMID:28953929 PMCID: PMC5617211.
  • Stemig, M., Astleford, K., Emery, A., Cho, J.J., Allen, B., Tsang-hai, H., Gopalakrishnan, R., Mansky, K.C., and Jensen, E. Deletion of Histone Deacetylase 7 in Osteoclasts Decreases Bone Mass in Mice by Interactions with MITF. 2015 PLOS ONE, Apr 15;10(4): e0123843 PMID: 25875108 PMCID: PMC4398560.
  • Pham, L., Kaiser, B., Rosa, A., Schwarz, T., Gopalakrishnan, R., Jensen, E.D. and Mansky, K.C. 2011. HDAC3 and HDAC7 Have Opposite Effects on Osteoclast Differentiation. Journal of Biological Chemistry 286(14): 12056-65. PMID: 21324898 PMCID: PMC3069409

Teaching

Teaching Areas

Basic Concepts in Skeletal and Craniofacial Development